Electronic Theses and Dissertations

Date of Award

2019

Document Type

Thesis

Degree Name

M.S. in Pharmaceutical Science

Department

Pharmaceutics and Drug Delivery

First Advisor

Soumyajit Majumdar

Second Advisor

Eman Ashour

Third Advisor

Michael Repka

Relational Format

dissertation/thesis

Abstract

Natamycin (NT) is a commercially available antifungal drug used for the treatment of fungal keratitis, an infection which affects the clear corneal surface and its associated layers. Currently, NT is available commercially as a 5% w/v ophthalmic suspension, to be administered topically. The main objective of the present investigation was to develop and evaluate the natamycin loaded nanosuspension (NT-NS), as well as it’s corresponding in-situ gel (NT-NS-GG), for the treatment of fungal keratitis. NT-NS was prepared using homogenization technique and optimized based on size, PDI, zeta potential (ZP), assay and process stability. Further, optimized NT-NS was modified into an in-situ gel with the addition of 0.2% gellan gum as a gelling agent and evaluated for rheological properties. in vitro release and trans corneal permeation studies were performed. Differential scanning calorimetry studies showed no interaction between the drug and other excipients being formulated into a NS. Particle size, PDI, ZP and assay of the optimized NT-NS formulation (formulation LP1) were 586. ± 61.3, 0.447 ± 0.12, -38.97 and 107 ± 0.6%, respectively. LP1 formulation was stable for up to 4 weeks under refrigerated and at room temperature conditions. in-situ gels showed satisfactory rheological properties, prolonged drug release and permeation through rabbit corneas. The results suggest that NT loaded NS could be an alternative topical ocular dosage form.

Available for download on Tuesday, September 22, 2020

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