Honors Theses

Date of Award

2018

Document Type

Undergraduate Thesis

Department

Chemistry and Biochemistry

First Advisor

Yu-Dong Zhou

Relational Format

Dissertation/Thesis

Abstract

One of the hallmarks of cancer is its ability to adapt its metabolism to survive and thrive despite needing to overcome significant energy barriers and low oxygen settings in order to do so. The hypoxia-inducible factor proteins (HIFs) play important roles in the processes in which cancer fulfills these requirements. The O-linked -N-acetylglucoseamine (O-GlcNAc) can be attached to cellular proteins through O-glycosylation, and is thus considered a potential target for anticancer mechanisms. HIFs are composed of (HIF-1 and HIF-2, respectively) and subunits. This research found that O-GlcNAc is able to attach to and modify the HIF proteins. This was accomplished through the use of immunoprecipitation followed by western blot, examining nuclear extract samples prepared from human breast cancer T47D cells. The O-GlcNAc was found to attach to both HIF-1 and HIF-2, and was most prominently expressed in the presence of O-(2-Acetamido-2-deoxy-D-glucopyranosylidenamino) N-phenylcarbamate (PUGNAc), which enhances O-GlcNAcylation by inhibiting the enzyme OGA.

Included in

Chemistry Commons

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.