Honors Theses

Date of Award

5-7-2019

Document Type

Undergraduate Thesis

Department

Chemistry and Biochemistry

First Advisor

Saumen Chakraborty

Relational Format

Dissertation/Thesis

Abstract

Nanoclusters are small nanoparticles that usually range around a micrometer in diameter. Gold Nanoclusters are known for their unique size and surface qualities and do not share metallic properties with nanoparticles or their respective bulk material. Some metal nanoclusters show high fluorescence and can be synthesized with protein mediation. Nanocluster formation depends on a number of variables, including temperature, pH, and type of reducing agent. This experiment was done to begin to understand how these variables may affect synthesis and what exactly they are. Pure Wild Type CSP-1 protein was induced, transformed, and purified in order to mediate gold and platinum nanocluster synthesis in a variety of different situations. When mixed in solution, the free Au3+ ions from HAuCl4 form clusters by attaching to the many cysteine side chains of the protein. Data was collected showing that in a neutral pH, using a solution of 50mM Tris and 150mM NaCl, highly fluorescent gold nanoclusters formed. These nanoclusters synthesized using 1M sodium hydroxide reducing agent showed the brightest fluorescence, a bright red-orange. Nanocluster formation in this experiment was confirmed through a matrix assisted laser desorption/ionization test. It was found that in the samples showing fluorescence, gold nanoclusters could be observed with a mass of 4kDa. This means that a nanocluster formed with around 20 Au atoms.

Included in

Biochemistry Commons

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.