Date of Award
Chemistry and Biochemistry
Kanglaite (KLT) is an anticancer herbal medicine developed in China that consists of mainly fatty acid triglycerides from Coix lacryma-jobi L. var. mayuen (Roman.) Stapf. In this study, we analyzed the effects of KLT on normal versus breast cancer cell metabolism using the C2C12, MCF-7, and MDA-MB-231 cell lines. Each cell line was divided into controls (n=3), cells with 1 μM of rotenone (n=2), or cells 0.3%, 1%, and 1.72% KLT concentrations. Cells were cultured in serum containing regular FCS and serum containing charcoal-stripped FCS in order to examine the full effects of KLT on the cell. Cell energy phenotype assays were conducted using the Seahorse XFe96 Analyzer, which simultaneously measured the cellular oxygen consumption rate (OCR) and extracellular acidification rate (ECAR). The data from the cell phenotype assay was then analyzed in order to determine the effect of KLT on breast cancer cell metabolism by comparing OCR and ECAR within and between cell lines and generating cell energy phenotypes. The results showed that KLT increased OCR and ECAR for C2C12 cells when cultured in charcoal-stripped fetal calf serum (FCS)-enriched media. The KLT increased OCR and ECAR in MCF-7 cells when cultured in regular FCS. The KLT increased OCR in MDA-MB-231 cells cultured in both serums and decreased ECAR in MDA-MB-231 cells cultured in regular FCS-enriched media.
Hillhouse, Sarah A., "Effects of Kanglaite, An Anticancer Herbal Medicine, on Breast Cancer Cell Metabolism" (2019). Honors Theses. 1057.