Honors Theses

Date of Award

Spring 5-8-2020

Document Type

Undergraduate Thesis

Department

Chemistry and Biochemistry

First Advisor

Yu-Dong Zhou

Second Advisor

Dale George Nagle

Relational Format

Dissertation/Thesis

Abstract

Histone deacetylase enzymes modify epigenetic characteristics of a genome by removing acetyl groups from histone proteins in chromatin. Histone deacetylase inhibitors work by stopping this activity which can have various results in a cell including apoptosis, cell cycle arrest, differentiation, and migration. The purpose of these experiments was to see how largazole, a histone deacetylase inhibitor, affected cell viability for breast cancer and associated metastatic cell lines in both normoxic and hypoxic conditions. The experiment was completed by setting up two 96-well plates with varying concentrations of largazole and conducting a sulforhodamine viability assay. The specific cell lines used in the experiment were MCF-7, MDA-MB-231, MCF-7 BoM, and MDA-MB-231 BoM. Largazole showed promise in treating triple-negative breast cancer, triple-negative breast cancer metastasis, and estrogen receptor-positive breast cancers.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

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