Honors Theses

Date of Award

Spring 5-4-2022

Document Type

Undergraduate Thesis

Department

Chemistry and Biochemistry

First Advisor

Randy Wadkins

Relational Format

Dissertation/Thesis

Abstract

The overall goal of this thesis was to investigate the environmental conditions that induce i-Motif folding of the DNA strand DUX4L22, a cytosine-rich segment of DNA found in the human genome. Cellular conditions were simulated using sodium cacodylate buffer and multiple weights of polyethylene glycol as a crowding agent. The presence of i-motifs were confirmed by Circular Dichroism spectroscopy. I found that DUX4L22 does form i-motifs under these physiological simulations at both acidic and neutral pHs. DUX4L22 therefore shows potential for use in studies of a wide variety of biotechnological advances, such as regulatory switches in nanomachines or drug-delivery systems.

Creative Commons License

Creative Commons Attribution-NonCommercial 4.0 International License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License

Available for download on Thursday, May 04, 2023

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Biochemistry Commons

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