Faculty and Student Publications
Document Type
Article
Publication Date
4-1-2022
Abstract
Oxygen levels in vivo are autonomously regulated by a supply–demand balance, which can be altered in disease states. However, the oxygen levels of in vitro cell culture systems, particularly microscale cell culture, are typically dominated by either supply or demand. Further, the oxygen microenvironment in these systems is rarely monitored or reported. Here, a method to establish and dynamically monitor autonomously regulated oxygen microenvironments (AROM) using an oil overlay in an open microscale cell culture system is presented. Using this method, the oxygen microenvironment is dynamically regulated via the supply–demand balance of the system. Numerical simulation and experimental validation of oxygen transport within multi-liquid-phase, microscale culture systems involving a variety of cell types, including mammalian, fungal, and bacterial cells are presented. Finally, AROM is applied to establish a coculture between cells with disparate oxygen demands—primary intestinal epithelial cells (oxygen consuming) and Bacteroides uniformis (an anaerobic species prevalent in the human gut).
Relational Format
journal article
Recommended Citation
Li, C., Humayun, M., Walker, G. M., Park, K. Y., Connors, B., Feng, J., Pellitteri Hahn, M. C., Scarlett, C. O., Li, J., Feng, Y., Clark, R. L., Hefti, H., Schrope, J., Venturelli, O. S., & Beebe, D. J. (2022). Under‐oil autonomously regulated oxygen microenvironments: A goldilocks principle‐based approach for microscale cell culture. Advanced Science, 9(10), 2104510. https://doi.org/10.1002/advs.202104510
DOI
10.1002/advs.202104510
Accessibility Status
Searchable text