Date of Award
Ph.D. in Pharmaceutical Sciences
Mitchell A. Avery
We examined fungal endophytes for their ability to inhibit bacterial, fungal and protozoal pathogens. Once antimalarial activity was discovered and confirmed, we sought to isolate and identify the active constituent(s) of our extracts. Bioassay-guided fractionation techniques were used to isolate our active compound, the structure of which was elucidated using 1H- and 13C-NMR. 2D-NMR and LC-MS analyses helped confirm that our endophytes were indeed producing artemisinin. Growth versus production studies were conducted to optimize our growth and extraction conditions while several other variables, like temperature and culture medium, were evaluated in an effort to improve our yields. Artemisinin production under both light and dark conditions was evaluated to determine if our production was the result of enzyme activity. In addition, labeling studies were conducted using 13C-labeled glucose.
Lee, Andrekeus, "Discovery, Isolation, and Identification of Antimalarials from Fungal Endophytes" (2012). Electronic Theses and Dissertations. 1449.
Emphasis: Medicinal Chemistry