Electronic Theses and Dissertations

Date of Award

1-1-2020

Document Type

Thesis

Degree Name

M.S. in Pharmaceutical Science

First Advisor

Michael A Repka

Second Advisor

Michael A Repka

Third Advisor

Mahavir Chougule

School

University of Mississippi

Relational Format

dissertation/thesis

Abstract

Curcumin has been known to have a variety of biological activities in different diseases, including inflammation, cancer, and diabetes. The limited bioavailability of curcumin is due to its low solubility and extensive metabolism, rendering curcumin unusable as an oral therapeutic agent. The aim of the study is to enhance the solubility and bioavailability of curcumin by preparing curcumin-piperine solid dispersion (SD) utilizing the hot-melt extrusion technique. Three physical mixtures with different ratios were prepared as following: F1 (5% w/w piperine, 15% w/w curcumin, 80% w/w Soluplus®), F2 (10% w/w piperine, 30% w/w curcumin, 60% w/w Soluplus®), and F3 (15% w/w piperine, 45% w/w curcumin, 40% w/w Soluplus®). The extrusion process was conducted using two different extrusion temperatures, 130 °C and 140 °C, to study the effect of temperature on physicochemical properties. Differential scanning calorimetry (DSC) was used to obtain the thermal characterization of the extrudates with a heating rate of 10 °C/min, ranging 20 to 200 °C. Additionally, Fourier transform infrared spectroscopy (FTIR) was performed to evaluate the chemical interaction between the components. Surface morphologies of the extrudates were analyzed utilizing the scanning electron microscope. Drug release studies for the pure compounds and the extrudates were performed using the USP apparatus II, and the samples were analyzed using high-performance liquid chromatography (HPLC). Curcumin-piperine solid dispersion was successfully prepared by the hot-melt extrusion technology. DSC results showed the presence of endothermic peaks, one for curcumin at 179 °C, and another for piperine at 132 °C. However, these peaks were not observed in the extrudates’ thermograms, indicating the solubilization of curcumin and piperine within the polymeric carrier at an amorphous state. The formation of hydrogen bonds between the components was observed in the FTIR spectra. Results from drug release studies showed enhancements in curcumin release profiles for up to 9-folds in the formulations, compared to pure curcumin.

Available for download on Tuesday, August 31, 2021

Share

COinS