Electronic Theses and Dissertations

Date of Award

1-1-2020

Document Type

Thesis

Degree Name

M.S. in Pharmaceutical Science

First Advisor

Soumyajit Majumdar

Second Advisor

Walter G Chambliss

Third Advisor

Eman Ashour

School

University of Mississippi

Relational Format

dissertation/thesis

Abstract

Corneal wound healing is a complex process involving cell death, migration, proliferation, differentiation, extracellular matrix remodeling and a various of growth factors. Of these factors, interleukin-1 and transforming growth factor beta allow the effectiveness of cell migration and tissue repair. Current management advocates multiple therapies aimed to prevent inflammation, avoid the fibrosis. Pirfenidone (PFD) has recently been investigated for its anti-fibrotic effect. The current study was undertaken to develop and optimize PFD-loaded nanoemulsion (NE; PFD-NE) which could increase the drug load and the duration of activity. Hot homogenization and probe sonication methods were used for the preparation of PFD-NEs, sesame oil and soybean oil as the oil part, Tween 80 and Poloxamer 188 as surfactants. Physicochemical properties of PFD-NE formulations were characterized such as particle size, polydispersity index, zeta potential and assay. The physical and chemical stability of the optimized PFD-NEs were observed under refrigerated and room temperature for one month. PFD-NE formulations showed desirable physical properties and observed to be stable for one month. Furthermore, in-vitro release studies of optimized PFD-NE formulations were performed in comparison with PFD solution (PFD-C) as control. From the release studies, a sustained release of the PFD was observed in PFD-NE formulations compared to PFD-C. Generally, the PFD-NE formulations showed great potential as an alternative delivery system for corneal wound healing treatment.

Available for download on Wednesday, August 31, 2022

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