Electronic Theses and Dissertations

Date of Award

1-1-2021

Document Type

Dissertation

Degree Name

Ph.D. in Pharmaceutical Sciences

First Advisor

Soumyajit Majumdar

Second Advisor

Michael Repka

Third Advisor

Samir Ross

Relational Format

dissertation/thesis

Abstract

Primaquine (PQ)-loaded solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC), and nano-emulsion (NE) were developed to investigate the influence of lipid excipients, surfactants, and drug loading on the physicochemical properties of lipid formulations. To investigate the drug release mechanisms from the lipid-based formulations, empirical release kinetic models such as zero-order, Higuchi, Korsmeyer-Peppas, and Hixson-Crowell were used. In an ex vivo hemolysis toxicity study, the efficacy of SLN, NLC, and NE to protect erythrocytes against PQ-induced cell lysis was investigated. The results show that all lipid formulations reduced the erythrocyte hemolysis by approximately 4.5-fold compared to the drug solution, suggesting that SLN, NLC, and NE have the potential to protect erythrocytes from PQ-induced cell lysis.Ciprofloxacin was used as a model drug to assess the effect of critical material attributes on lipid formulation characteristics. A six-factor, two-level minimum run resolution IV (MR4) screening design was used to identify the significant main effects and low-order interactions. Finally, an at-home-applicable, customized, and rapidly detachable transparent eye patch with hydrogel-forming microneedle arrays was developed. The microneedle eye patch can be easily applied on the surface of the cornea and bulbar conjunctiva with a gentle press by fingertip. The drug release and effects of CIP-MNs on the ocular tissues were investigated. Results show that nearly 69-100% of CIP loaded in MNs permeated across rabbit cornea and sclera in 4 hours, compared to cornea treated with CIP market formulation (16% permeation).

Concentration/Emphasis

Pharmaceutical sciences

Available for download on Friday, February 17, 2023

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