Honors Theses
Date of Award
Spring 5-5-2022
Document Type
Undergraduate Thesis
Department
School of Pharmacy
First Advisor
James Stewart
Relational Format
Dissertation/Thesis
Abstract
Diabetes is one of the largest health concerns in the United States. Its continuous increase in prevalence has led to it being one of the largest healthcare costs and consistently listed as one of the leading causes of death. That being said, diabetes also increases the risk of other health conditions, one of which is cardiovascular disease. Left ventricle hypertrophy resulting in the stiffening of the left ventricle and diastolic dysfunction is one form of cardiovascular disease associated with diabetes. Thought to be impacted by decreased fibroblast migration in the extracellular matrix, the experiments performed in this study seek to review components believed to influence migration including the AGE/RAGE signaling cascade and glucose media concentration. Using cardiac fibroblasts isolated from diabetic and non-diabetic mice with an intact AGE/RAGE cascade as well as diabetic and non-diabetic mice with an impaired AGE/RAGE cascade the number of migrated fibroblasts was totaled with varying amounts of AGE and glucose concentration. The results of the study showed that diabetic RAGE knockout cells had the greatest number of migrated fibroblasts, migration increased as AGE volumes increased, and that glucose media concentration had no impact on fibroblast migration. This signifies that the AGE/RAGE cascade does influence the migration of fibroblasts. Furthermore, it appears as though cardiac tissue isolated from various genotypes maintain their in vivo qualities when cultured using different concentrations of glucose.
Recommended Citation
Kanauss, Lorral, "The Effect of AGE Concentration and Glucose Levels in RAGE-Mediated Cardiac Fibroblast Migration in Type 2 Diabetes" (2022). Honors Theses. 2676.
https://egrove.olemiss.edu/hon_thesis/2676
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This work is licensed under a Creative Commons Attribution 4.0 International License.