Identification of Signal Transducers in Plant Shoot Gravitropism using Genetic Screens

Author

Lilla ReedFollow

Date of Award

Spring 5-10-2023

Document Type

Undergraduate Thesis

Department

Biology

First Advisor

Yongjian Qiu

Second Advisor

Stephen Brewer

Third Advisor

Jason Hoeksema

Relational Format

Dissertation/Thesis

Abstract

Gravitropism is an essential response for plant growth and architecture. The signal transduction pathway that links gravity sensing to gravity response remains unclear because the key signal transducers involved in this pathway are unknown. In this project, we applied an artificial microRNA(amiRNA)-assisted genetic screen in isolating mutants displaying altered gravitropic phenotype in the darkness. Two amiRNA lines showed a significantly decreased gravitropic phenotype in the hypocotyl, and amiRNAs in these lines were identified to target a hypothetical transmembrane protein and a receptor kinase, respectively. Further experiments need to be done to confirm their phenotypes and establish them as signal transducers in the gravitropic pathway.

Recommended Citation

Reed, Lilla, "Identification of Signal Transducers in Plant Shoot Gravitropism using Genetic Screens" (2023). Honors Theses. 2991.
https://egrove.olemiss.edu/hon_thesis/2991

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This work is licensed under a Creative Commons Attribution 3.0 License.