Honors Theses

Date of Award

2018

Document Type

Undergraduate Thesis

Department

Biology

First Advisor

Sarah Liljegren

Relational Format

Dissertation/Thesis

Abstract

Understanding the molecular controls involved in plant development is important in understanding plant physiology as a whole, and this knowledge in turn can lead to useful applications in agriculture and industry. This project investigates the roles of two transcription factors, SHOOT MERISTEMLESS (STM) and ARABIDOPSIS THALIANA HOMEOBOX GENE1 (ATH1) in the molecular pathway that creates boundaries between organs in flowers of Arabidopsis thaliana. Mutations in the STM and ATH1 genes prevent formation of basal boundaries between the floral organs and the stem, causing abscission defects in flowers. If the STM and ATH1 genes are also critical for establishing the boundaries between individual floral organs, I expected to see fusion events in mutant flowers. Since STM is involved in maintaining the stem cell populations in shoot and flower meristems, I expected to see stm mutant flowers with fewer organs. If the STM and ATH1 genes have redundant roles in inter-organ boundary formation and SAM maintenance, I expected to see significantly fewer floral organs and more severe fusion phenotypes in double mutant plants. I found that the mutant plants indeed had fusion defects, and these were most commonly found between organs of the same type. Fusion defects and absence of organs were most severe in double mutant plants, suggesting that the ATH1 and STM genes are functionally redundant in these pathways.

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