Honors Theses

Date of Award

2015

Document Type

Undergraduate Thesis

Department

Chemistry and Biochemistry

First Advisor

Murrell Godfrey

Relational Format

Dissertation/Thesis

Abstract

The purpose of this research was to develop a method for the detection and quantitation of caffeine and 11-nor-9-carboxy-Δ9- tetrahydrocannabinol in fingerprints using matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). The matrix used was 2,5-dihydroxybenzoic acid (DHB). The presence of the analytes was confirmed by analysis using liquid chromatography-mass spectrometry (LC-MS). 11-nor-9-carboxy-Δ9- tetrahydrocannabinol was chosen as the drug of interest because it is the major metabolite of tetrahydrocannabinol, the main psychoactive component of marijuana. Marijuana's popularity as a recreational drug continues to increase with its legalization and decriminalization across the country. It is critical to establish methods for the quantification of the drug in humans for the purpose of determining levels of intoxication, similar to measuring blood alcohol levels. This research shows that MALDI-MS is not capable of quantification at this time, only qualitation within the limits of detection of the instrument. Further studies will explore better methods to improve quantification and new matrices to improve the limit of detection.

Accessibility Status

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Included in

Chemistry Commons

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