Honors Theses

Date of Award

2018

Document Type

Undergraduate Thesis

Department

Biology

First Advisor

Bradley Jones

Relational Format

Dissertation/Thesis

Abstract

Genetic screens are performed in order to characterize genes and mutations, often relating to developmental processes. A previous genetic screen performed in Drosophila melanogaster found three mutations on the second chromosome affecting glial cell development: FF12, DD68, and D19. These mutations each disrupted the pattern and expression of reversed polarity (repo), a target gene of the glial fate master regulator, glial cells missing (gcm). Here, I use recombination mapping with phenotypically observable dominant markers to locate the genetic map position of each mutation. This method provides a rapid estimate of the mutations' locations that can then be tested against molecularly-defined deficiencies to pin-point a precise position. FF12, DD68, and D19 were all determined to be on chromosome arm 2R.

Accessibility Status

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Biology Commons

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