Date of Award
Genetic screens are performed in order to characterize genes and mutations, often relating to developmental processes. A previous genetic screen performed in Drosophila melanogaster found three mutations on the second chromosome affecting glial cell development: FF12, DD68, and D19. These mutations each disrupted the pattern and expression of reversed polarity (repo), a target gene of the glial fate master regulator, glial cells missing (gcm). Here, I use recombination mapping with phenotypically observable dominant markers to locate the genetic map position of each mutation. This method provides a rapid estimate of the mutations' locations that can then be tested against molecularly-defined deficiencies to pin-point a precise position. FF12, DD68, and D19 were all determined to be on chromosome arm 2R.
Coleman, Zane Bryant, "Determination of the Chromosomal Position of Three Mutations Using Recombination Mapping in Drosophila Melanogaster" (2018). Honors Theses. 978.