Posters and Spotlights
Purification of Lectins from different legume seeds (Fabaceae) by PEG 600 –ATPS and their Characterization using Mass Spectrometry
Start Date
30-4-2025 11:30 AM
Document Type
Event
Description
Poster Presenter: Iffat Parveen
Research Team: Iffat Parveen, Sandeep Misra, Joshua Sharp, Ikhlas A. Khan
Abstract: Lectins are glycoproteins that can bind reversibly to different carbohydrates and hence used as defense mechanism against pathogens and pests in plants. Due to their sugar binding properties lectins have been explored in various physiological processes and found to have insecticidal action, antibacterial effects and antifungal properties, antitumorous, and analgesic properties in humans. Legumes are the major source of lectins among plants. However, they cause anti-nutritional effects like gastro-intestinal problems if ingested unprocessed or undercooked. Food safety is a major concern these days and therefore, the legume lectins are being studied in detail. Traditionally lectins were purified using various protein purification methods and chromatography techniques that are both labor and cost intensive and time consuming. In the present work, lectins were purified from different species of legumes using a twostep method called PEG 600–Ammonium-Sulfate Aqueous Two-Phase System (ATPS) and were characterized by Mass Spectrometry (LC-MS/MS). These techniques provide a valuable tool for rapid purification and identification of antinutritional factors such as lectins from commonly consumed foods and may help in investigating food poisoning or outbreaks related to legume or plant protein products.
Relational Format
poster
Recommended Citation
Parveen, Iffat, "Purification of Lectins from different legume seeds (Fabaceae) by PEG 600 –ATPS and their Characterization using Mass Spectrometry" (2025). Showcase of Research and Scholarly Activity. 40.
https://egrove.olemiss.edu/ored_showcase/2025/posters/40
Purification of Lectins from different legume seeds (Fabaceae) by PEG 600 –ATPS and their Characterization using Mass Spectrometry
Poster Presenter: Iffat Parveen
Research Team: Iffat Parveen, Sandeep Misra, Joshua Sharp, Ikhlas A. Khan
Abstract: Lectins are glycoproteins that can bind reversibly to different carbohydrates and hence used as defense mechanism against pathogens and pests in plants. Due to their sugar binding properties lectins have been explored in various physiological processes and found to have insecticidal action, antibacterial effects and antifungal properties, antitumorous, and analgesic properties in humans. Legumes are the major source of lectins among plants. However, they cause anti-nutritional effects like gastro-intestinal problems if ingested unprocessed or undercooked. Food safety is a major concern these days and therefore, the legume lectins are being studied in detail. Traditionally lectins were purified using various protein purification methods and chromatography techniques that are both labor and cost intensive and time consuming. In the present work, lectins were purified from different species of legumes using a twostep method called PEG 600–Ammonium-Sulfate Aqueous Two-Phase System (ATPS) and were characterized by Mass Spectrometry (LC-MS/MS). These techniques provide a valuable tool for rapid purification and identification of antinutritional factors such as lectins from commonly consumed foods and may help in investigating food poisoning or outbreaks related to legume or plant protein products.