Posters and Spotlights
Glycoscience Center of Research Excellence (GlyCORE)
Start Date
30-4-2025 11:30 AM
Document Type
Event
Description
The Glycoscience Center of Research Excellence (GlyCORE) is a Phase I Center of Biomedical Research Excellence sponsored by the NIH. It was established in 2020 at the School of Pharmacy, University of Mississippi. GlyCORE comprises three core facilities: Analytical and Biophysical Chemistry Core, Computational Chemistry and Bioinformatics Core, and The Imaging Core. The GlyCORE Imaging Core offers a range of services such as live cell imaging, FRET, FLIP and FRAP photobleaching measurements, immuno- and lectin staining, SEM coupled with EDS, and other imaging and image processing services. GlyCORE houses state-of-the-art instruments such as Stereomicroscopy, Epi-fluorescent and Bright Field Microscopy, Imaging Flow Cytometer, and Field-Emission Scanning Electron Microscope (FESEM) with multiple detectors: Energy Dispersive X-Ray Spectrometer (EDS). Confocal Laser Scanning Microscopy (CLSM). The Core’s Leica SP8 CLSM has powerful multichannel capabilities with a resonant scanner (faster scanning) and HyD PMTs (super sensitive). It has an excitation of 405 nm, 442 nm, and a white light laser (WLL). The white light laser has continuous excitation lines from 470nm up to 670nm, and is pulsatile with a frequency of 80 Hz, allowing excitation to be tunable in 1 nm increments up to 8 laser lines simultaneously, such that almost any visible dye or fluorescent protein can be excited. This scope is highly sensitive, multispectral, and fast, enabling many advanced confocal techniques, such as FRET and FLIM. The JSM 7200FLV FESEM is another cutting-edge instrument found in the GlyCORE Imaging Core. The FESEM is highly versatile; it offers a new level of expanded performance using an in-lens Schottky Field emission gun. Advanced analysis of nanostructures and determining the elemental composition of the sample through X-ray spectroscopy (EDX) is possible. The FESEM enables the analysis of biological samples, geological materials, synthetic polymers, metal plating, nanoparticles, and nanocomposites. Magnification can go up to 1,000,000X, and resolution is 1.2 nm at 30 kV. Electron-transparent samples with sub 0.8 nm resolution using the STEM detector are also possible. The GlyCORE Imaging Core specializes in supporting glycoscience research and other advanced research in different fields of science.
Relational Format
report
Recommended Citation
Adams, Sebastian John, "Glycoscience Center of Research Excellence (GlyCORE)" (2025). Showcase of Research and Scholarly Activity. 89.
https://egrove.olemiss.edu/ored_showcase/2025/posters/89
Glycoscience Center of Research Excellence (GlyCORE)
The Glycoscience Center of Research Excellence (GlyCORE) is a Phase I Center of Biomedical Research Excellence sponsored by the NIH. It was established in 2020 at the School of Pharmacy, University of Mississippi. GlyCORE comprises three core facilities: href="https://olemiss.edu/glycore/analytical-biophysical-chemistry-research-core/"">Analytical and Biophysical Chemistry Core, Computational Chemistry and Bioinformatics Core, and The Imaging Core. The GlyCORE Imaging Core offers a range of services such as live cell imaging, FRET, FLIP and FRAP photobleaching measurements, immuno- and lectin staining, SEM coupled with EDS, and other imaging and image processing services. GlyCORE houses state-of-the-art instruments such as Stereomicroscopy, Epi-fluorescent and Bright Field Microscopy, Imaging Flow Cytometer, and Field-Emission Scanning Electron Microscope (FESEM) with multiple detectors: Energy Dispersive X-Ray Spectrometer (EDS). Confocal Laser Scanning Microscopy (CLSM). The Core’s Leica SP8 CLSM has powerful multichannel capabilities with a resonant scanner (faster scanning) and HyD PMTs (super sensitive). It has an excitation of 405 nm, 442 nm, and a white light laser (WLL). The white light laser has continuous excitation lines from 470nm up to 670nm, and is pulsatile with a frequency of 80 Hz, allowing excitation to be tunable in 1 nm increments up to 8 laser lines simultaneously, such that almost any visible dye or fluorescent protein can be excited. This scope is highly sensitive, multispectral, and fast, enabling many advanced confocal techniques, such as FRET and FLIM. The JSM 7200FLV FESEM is another cutting-edge instrument found in the GlyCORE Imaging Core. The FESEM is highly versatile; it offers a new level of expanded performance using an in-lens Schottky Field emission gun. Advanced analysis of nanostructures and determining the elemental composition of the sample through X-ray spectroscopy (EDX) is possible. The FESEM enables the analysis of biological samples, geological materials, synthetic polymers, metal plating, nanoparticles, and nanocomposites. Magnification can go up to 1,000,000X, and resolution is 1.2 nm at 30 kV. Electron-transparent samples with sub 0.8 nm resolution using the STEM detector are also possible. The GlyCORE Imaging Core specializes in supporting glycoscience research and other advanced research in different fields of science.