Box–Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential

Authors

Ajaz Ahmad, King Saud University
Muneeb U. Rehman, King Saud University
Adil Farooq Wali, Ras Al Khaima Medical and Health Sciences University (RAK)
Hamed A. El-Serehy, King Saud University
Fahad A. Al-Misned, King Saud University
Saleh N. Maodaa, King Saud University
Hossam M. Aljawdah, King Saud University
Tahir Maqbool Mir, University of Mississippi, Research Institute Pharmaceutical Science
Parvaiz Ahmad, King Saud University

Document Type

Article

Publication Date

9-1-2020

Abstract

© 2020 by the authors. In the present investigation, the ultrasound-assisted extraction (UAE) conditions and optimization of Rhododendron arboreum polysaccharide (RAP) yield were studied by a Box–Behnken response surface design and the evaluation of its antioxidant potential. Three parameters that affect the productivity of UAE, such as extraction temperature (50–90 ◦C), extraction time (10–30 min), and solid–liquid ratio (1–2 g/mL), were examined to optimize the yield of the polysaccharide percentage. The chromatographic analysis revealed that the composition of monosaccharides was found to be glucose, galactose, mannose, arabinose, and fucose. The data were fitted to polynomial response models, applying multiple regression analysis with a high coefficient of determination value (R2 = 0.999). The data exhibited that the extraction parameters have significant effects on the extraction yield of polysaccharide percentage. Derringer’s desirability prediction tool was attained under the optimal extraction conditions (extraction temperature 66.75 ◦C, extraction time 19.72 min, and liquid–solid ratio 1.66 mL/g) with a desirability value of 1 yielded the highest polysaccharide percentage (11.56%), which was confirmed through validation experiments. An average of 11.09 ± 1.65% of polysaccharide yield was obtained in optimized extraction conditions with a 95.43% validity. The in vitro antioxidant effect of polysaccharides of R. arboreum was studied. The results showed that the RAP extract exhibited a strong potential against free radical damage.

Relational Format

journal article

Recommended Citation

Ahmad, A., Rehman, M. U., Wali, A. F., El-Serehy, H. A., Al-Misned, F. A., Maodaa, S. N., Aljawdah, H. M., Mir, T. M., & Ahmad, P. (2020). Box–Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential. Molecules, 25(17), 3835. https://doi.org/10.3390/molecules25173835

DOI

10.3390/molecules25173835