Decaffeinated green tea extract does not elicit hepatotoxic effects and modulates the gut microbiome in lean B6C3F₁ mice

Authors

Bill J. Gurley, University of Arkansas for Medical Sciences
Isabelle R. Miousse, University of Arkansas for Medical Sciences
Intawat Nookaew, University of Arkansas for Medical Sciences
Laura E. Ewing, University of Arkansas for Medical Sciences
Charles M. Skinner, University of Arkansas for Medical Sciences
Piroon Jenjaroenpun, University of Arkansas for Medical Sciences
Thidathip Wongsurawat, University of Arkansas for Medical Sciences
Stefanie Kennon-McGill, University of Arkansas for Medical Sciences
Bharathi Avula, University of Mississippi School of Pharmacy
Ji Yeong Bae, University of Mississippi School of Pharmacy
Mitchell R. McGill, University of Arkansas for Medical Sciences
David Ussery, University of Arkansas for Medical Sciences
Ikhlas A. Khan, University of Mississippi School of Pharmacy
Igor Koturbash, University of Arkansas for Medical Sciences

Document Type

Article

Publication Date

4-1-2019

Abstract

© 2019 The Author(s) The aim of this study is the development of validated HPTLC method for the quantification of vitexin from Passiflora foetida commercial herbal formulations. The developed method was validated, in accordance with ICH guidelines for precision, accuracy, specificity and robustness. The plate was developed using ethyl acetate:methanol:water:formic acid 30:4:2:1(%, v/v/v/v) on 20 × 10 cm glass coated silica gel 60 F254 plates and the developed plate was scanned and quantified densitometrically at λ = 340 nm. Linear regression analysis revealed a good linear relationship between peak area and amount of vitexin in the range of 100–700 ng/spot. The amount of vitexin in nine commercial herbal formulations was successfully quantified by the developed HPTLC method. The developed and validated high performance thin layer chromatographic method offers a new sensitive and reliable tool for quantification of vitexinin in various herbal formulations containing Passiflora foetida.

Relational Format

journal article

Recommended Citation

Gurley, B. J., Miousse, I. R., Nookaew, I., Ewing, L. E., Skinner, C. M., Jenjaroenpun, P., Wongsurawat, T., Kennon-McGill, S., Avula, B., Bae, J.-Y., McGill, M. R., Ussery, D., Khan, I. A., & Koturbash, I. (2019). Decaffeinated Green Tea Extract Does Not Elicit Hepatotoxic Effects and Modulates the Gut Microbiome in Lean B6C3F1 Mice. Nutrients, 11(4), 776. https://doi.org/10.3390/nu11040776

DOI

10.3390/NU11040776