Electronic Theses and Dissertations

Date of Award

2015

Document Type

Thesis

Degree Name

M.A. in Psychology

Department

Psychology

First Advisor

Kenneth J. Sufka

Second Advisor

Todd A. Smitherman

Third Advisor

Michael Allen

Relational Format

dissertation/thesis

Abstract

Endocannabinoid modulation of cancer-related pain is well-documented. Sativex, a cannabinoid extract with a 1:1 ratio of tetrahydrocannabinol (thc) and cannabidiol (cbd) has been shown to alleviate neuropathic pain associated with chemotherapy. This research examined whether thc or cbd alone is effective in attenuating or preventing tactile allodynia associated with cisplatin-administration. Mice (c57bl/6) were given eight doses of 2.3 mg/kg cisplatin or saline solution ip every second day to induce tactile allodynia (ringers on alternate days). Tactile responses to hind-paws were quantified in g of force using an electric von frey (evf) prior to (baseline) and after the cisplatin administration protocol. Separate groups of mice were then given vehicle, 100 mg/kg gabapentin, 2 mg/kg thc or 2 mg/kg cbd ip and tested 60 m later on evf. In the prevention studies, cbd (0.0, 0.5,1.0, and 2.0 mg/kg) or thc (0.0, 0.5,1.0, and 2.0 mg/kg) was given ip 30 m prior to cisplatin administration (2.3 or 1.0 mg/kg) utilizing a six-dose alternate day protocol. As before, tactile response was measured using evf prior to and after cisplatin dosings. Cisplatin produced a reduction in g of force indicative of neuropathy in each study. Gabapentin, thc, and cbd did not alter tactile responses in control mice. Cisplatin allodynia was attenuated by gabapentin, thc, and cbd but was not prevented by either cannabinoid tested. These data demonstrate that thc and cbd administered alone, unlike that in sativex, can achieve analgesic effects in this murine model of cisplatin neuropathy.

Concentration/Emphasis

Emphasis: Experimental Psychology

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