Electronic Theses and Dissertations

Date of Award

1-1-2014

Document Type

Dissertation

Degree Name

Ph.D. in Pharmaceutical Sciences

Department

Biomolecular Sciences

First Advisor

Michael A. Repka

Second Advisor

John O'Haver

Third Advisor

Seongbong Jo

Relational Format

dissertation/thesis

Abstract

Soluplus® (SOL), a graft amorphous copolymer, composed of polyethylene glycol, vinyl acetate and vinylcaprolactam in a ratio of 13: 30: 57, was utilized to prepare solid dispersions containing felodipine (FEL) or ketoconazole (KTZ) using hot-melt extrusion technology. The melting point depression approach was utilized to determine the miscibility and solubility of the model compounds within Soluplus® , of which felodipine demonstrated higher solubility when compared to ketoconazole (14% vs 4.3% w/w) at room temperature (298K). Moreover, the solubility parameters of FEL, KTZ and SOL were calculated as 21.76, 26.51 and 21.64, respectively.

Polarized light microscopy, Fourier transform infrared spectroscopy (FT-IR), Raman microscopy, differential scanning calorimetry (DSC), X-Ray diffraction (XRD), and scanning electron microscopy (SEM) were explored to characterize the FEL-SOL solid dispersions, and FEL was found to be molecularly dispersed in the matrix at a concentration of 10% w/w, which also demonstrated a higher solubility.

A central composite design (CCD) was applied to optimize the processing parameters for KTZ-SOL solid dispersions and the final formulation containing 29.8% drug was extruded at a temperature of 140°C and screw speed of 31 rpm. The robustness of the design was also examined.

A solid dispersion system of paclitaxel (PTX) was also developed to increase the aqueous solubility in order to overcome the side effects of its commercial products Taxol® , which was accomplished with the addition of a non-ionic surfactant, Cremophor® EL. PolyOx™ WSR N-80 (Molecular weight: 200,000 Da) was utilized as the matrix carrier, in which the concentration of PTX was determined as 30%. Various surfactants and solubilizers, including sodium lauryl sulfate (SLS), Lutrol® F68 (F68) and polyethylene glycol (PEG) 3350 were incorporated into the formulation. Of these, PEG 3350 was found to increase the solubility of PTX to 9.29 μg/ml (9-fold); however, the formulation started to precipitate after 2 hours due to the high energy amorphous state of PTX. 5% hydroxypropyl methylcellulose acetate succinate (HPMCAS-LF) successfully postponed the precipitation and maintained the solubility up to 12 hours by forming hydrogen bonds with PTX. This finding was confirmed by FT-IR analysis.

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