Date of Award
1-1-2021
Document Type
Thesis
Degree Name
M.S. in Pharmaceutical Science
First Advisor
Vitor H. Pomin
Second Advisor
Josh Sharp
Third Advisor
Paul Boudreau
Relational Format
dissertation/thesis
Abstract
Ganoderma lucidum is a polypore fungus commonly known as Lingzhi, Youngji or Reishi mushroom in East Asia. It is frequently marketed as an herbal supplement and often utilized as traditional Chinese medicine. Our research comprises the structural investigation of the principal polysaccharide from this fungus. After proper purification, the fungal glucan was subjected to multiple experiments of NMR spectroscopy, including 1D 1H and 13C, 2D; 1H-1H COSY, 1H-1H TOCSY, 1H-1H NOESY, 1H-13C HSQC, 1H-1H Phase Sensitive-TOCSY, and 1H-13C TOCSY-HSQC NMR spectra. Data has confirmed that this fungal polysaccharide contains residues of glucose (91.31%) as major, galactose (4.99%), and mannose (3.69%) as minor constituents in the glucan extract. Anomeric signals indicated that all these three residues occur in both ? and ? configurations. This fungal glucan is mostly composed of 6-linked units together with minor amounts of the 3-linked unit and exhibits both linear and branching forms.
Recommended Citation
Gonzales, Alysia, "NMR-Based characterization of a glucan isolated from Ganoderma lucidum" (2021). Electronic Theses and Dissertations. 2007.
https://egrove.olemiss.edu/etd/2007