Date of Award
1-1-2022
Document Type
Thesis
Degree Name
M.S. in Pharmaceutical Science
First Advisor
Michael Repka
Second Advisor
Walter Chambliss
Third Advisor
Eman Ashour
School
University of Mississippi
Relational Format
dissertation/thesis
Abstract
The purpose of the present study was to create Indocyanine Green (ICG) loaded liposomal formulations that were efficient and had controlled drug release. During the Second World War, the photographic dye indocyanine green (ICG) was created. ICG was developed as a medical diagnostic tool for cardiac output tests and for monitoring liver function after receiving FDA approval. The preparation of ICG-encapsulated conventional and PEGylated liposomes containing different types of phosphatidylcholine (PC) such as HSPC, DSPC, DPPC and cholesterol (CH) in the molar ratios of 55:45 and PC:CH: DSPE-PEG2K (polyethylene glycol) in the molar ratio of 55:40:5 respectively was done using thin-film hydration method. PEGylated and conventional liposomes containing ICG were prepared in this work, and their characteristics, including particle size, polydispersity index, encapsulation efficiency and in-vitro drug release were examined. Formulation (F3) which consist of DSPC lipid showed the maximum encapsulation efficiency of 82.15% ± 2.32% and the minimum particle size of 94.68 ± 3.12. To find out if the formulations are stable in terms of physical and chemical properties over a two-month period, stability experiments were also conducted. The release of ICG in vitro has been the subject of research. In vitro release study results indicated the sustained release of ICG from PEGylated liposomes. However, further studies including in vivo experiments are warranted.
Recommended Citation
Uttreja, Prateek, "Preparation, Characterization and In-Vitro Release Studies of ICG-Encapsulated Liposomes" (2022). Electronic Theses and Dissertations. 2462.
https://egrove.olemiss.edu/etd/2462
Concentration/Emphasis
Pharmaceutics