Electronic Theses and Dissertations

Date of Award

1-1-2023

Document Type

Thesis

Degree Name

M.S. in Pharmaceutical Science

First Advisor

Michael A. Repka

Second Advisor

Dr. Walter Chambliss

Third Advisor

Seongbong Jo

School

University of Mississippi

Relational Format

dissertation/thesis

Abstract

Solid Lipid Nanoparticles are first-generation colloidal carriers based on lipids, with their sizes ranging from 40-1000 nm. They are composed of biocompatible lipids, which are dispersed in an aqueous medium containing surfactant to form a lipid core. The active pharmaceutical ingredient is dispersed uniformly in the lipid matrix of the formulation. Water, along with lipids, is one of the major constituents in the preparation of solid lipid nanoparticles. Various active ingredients have adverse effects when taken by oral route and hence can be formulated as alternative drug delivery methods for the reduction of side effects. The objective of the present study was to develop and characterize solid lipid nanoparticles suitable for incorporating an anti-fungal drug, Fluconazole in solid lipid nanoparticles for formulation stability and reducing adverse effects associated with the oral administration of the drug.Fluconazole (FLZ) was selected as a model drug for preparing solid lipid nanoparticles consisting of Stearic acid and Precirol ATO 5 as the lipids, Tween 80 as the surfactant, and Polyvinyl alcohol as the stabilizer. Hot homogenization and probe sonication techniques were used for the development of the fluconazole solid lipid nanoparticle formulation. Developed SLNs were characterized for the following: Particle size, polydispersity index, zeta potential, entrapment efficiency, and in-vitro drug release. The in-vitro release of the best formulation also showed promising results with 92.13% release in 24 hrs., with initial burst release followed by a sustained release. The particle size, PDI, and zeta potential of the prepared formulations were found to be in the range of 336.4 to 401.5 nm, 0.216 to 0.312, and -21.8 to -28.9 mV respectively.

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