Document Type
Oral Presentation
Location
Oxford Convention Center, 102 Ed Perry Boulevard Oxford, MS 38655
Event Website
https://oxfordicsb.org/
Start Date
18-4-2024 11:00 AM
End Date
18-4-2024 11:15 AM
Description
Over time, the popularity and consumption of herbal medicine and its products like nutraceuticals, botanical drugs, and dietary supplements have increased immensely. Parallelly, several health issues associated with the overconsumption of these products have also been raised, which has attracted enormous attention. Herb-drug interaction (HDI) is a health issue caused by herbs or phytochemicals that modulate the homeostasis of drug-metabolizing enzymes (CYPs) and transporters, thus altering the pharmacokinetic of clinical drugs. To date, only a few medicinal herbs, such as Hypericum perforatum, Hydrastis canadensis, Glycyrrhiza glabra, and Camellia sinensis etc., were recognized as HDI perpetrators. However, many need to be yet identified. As part of our continuous efforts towards studying the safety of medicinal plants, we screened the hydroethanolic extracts of a large collection of herbs for interaction with xeno-receptors (PXR and AhR), drug metabolizing enzymes (CYP 3A4 and 1A2), and transporters (P-gp). We found several plants displaying potent agonism with PXR and increased its activity to 4 to 7-fold at 60 μg/mL while others increased AhR activity up to 10 to 40-fold at 30 μg/mL. We also found the extracts of several plants that strongly inhibited CYP3A4 or CYP1A2 activity. Selected plants were studied further in more advanced assays involving gene expression and enzyme activity in hepatocytes. The HDI of some herbs like Bulbine natalensis, Zingiber officinale, Phyllanthus amarus, Garcinia gummi-gutta and Pausinystalia johimbe were studied in detail and some are underway. Further studies of selected candidates that could be future perpetrators of HDI are warranted.
Recommended Citation
Khan, Ikhlas A.; Chittiboyina, Amar G.; Khan, Shabana; Gurley, Bill; and Husain, Islam, "Screening of medicinal herbs to predict the potential perpetrators of herb-drug interaction" (2024). Oxford ICSB. 25.
https://egrove.olemiss.edu/icsb/2024_ICSB/Schedule/25
Publication Date
April 2024
Accessibility Status
Searchable text
Included in
Screening of medicinal herbs to predict the potential perpetrators of herb-drug interaction
Oxford Convention Center, 102 Ed Perry Boulevard Oxford, MS 38655
Over time, the popularity and consumption of herbal medicine and its products like nutraceuticals, botanical drugs, and dietary supplements have increased immensely. Parallelly, several health issues associated with the overconsumption of these products have also been raised, which has attracted enormous attention. Herb-drug interaction (HDI) is a health issue caused by herbs or phytochemicals that modulate the homeostasis of drug-metabolizing enzymes (CYPs) and transporters, thus altering the pharmacokinetic of clinical drugs. To date, only a few medicinal herbs, such as Hypericum perforatum, Hydrastis canadensis, Glycyrrhiza glabra, and Camellia sinensis etc., were recognized as HDI perpetrators. However, many need to be yet identified. As part of our continuous efforts towards studying the safety of medicinal plants, we screened the hydroethanolic extracts of a large collection of herbs for interaction with xeno-receptors (PXR and AhR), drug metabolizing enzymes (CYP 3A4 and 1A2), and transporters (P-gp). We found several plants displaying potent agonism with PXR and increased its activity to 4 to 7-fold at 60 μg/mL while others increased AhR activity up to 10 to 40-fold at 30 μg/mL. We also found the extracts of several plants that strongly inhibited CYP3A4 or CYP1A2 activity. Selected plants were studied further in more advanced assays involving gene expression and enzyme activity in hepatocytes. The HDI of some herbs like Bulbine natalensis, Zingiber officinale, Phyllanthus amarus, Garcinia gummi-gutta and Pausinystalia johimbe were studied in detail and some are underway. Further studies of selected candidates that could be future perpetrators of HDI are warranted.
https://egrove.olemiss.edu/icsb/2024_ICSB/Schedule/25
Comments
This work is supported in part by the US Department of Food and Drug Administration (5U01FD004246) and the US Department of Agriculture, Agricultural Research Service (58-6060-6-015).