Document Type
Oral Presentation
Location
Oxford Conference Center, Oxford MS
Event Website
https://oxfordicsb.org/
Start Date
8-4-2025 12:00 PM
Description
This study aimed to determine if functional mushroom preparation supplementation with Cordyceps militaris mycelium and fruiting body, Ganoderma lucidum mycelium and fruiting body, and a blend of ten species of functional mushrooms could impact gut health. The Ex Vivo model Simulator of Human Intestinal Microbial Ecosystem (SHIME®) has been used to study the behaviour of a human microbiome in response to various factors. The aim of this study was to demonstrate changes in the microbiome after supplementation with functional mushroom preparation and the resulting neuroactive metabolome using the SHIME® model. The endpoints measured were microbial fermentation activity, metabolic fingerprinting, microbial community composition, barrier integrity (TEER) and inflammation (pro- and anti-inflammatory cytokines) and targeted metabolomics evaluation of the compounds produced by the microbiome in the samples. The results showed that functional mushroom supplementation did change the composition of the gut microbiota. The type of species enriched in each experiment were unique to each mushroom preparation pointing to the unique composition of each mushroom preparations beyond their fiber content. Supplementation with all mushroom preparations led to an increase in SCFAs. SCFAs play a pivotal role in homeostasis and physiology in the gut but also in other organs. The analysis of the metabolome revealed increase concentration of unique molecules for each mushroom preparations although concentrations of butyrobetaine and tryptamine were increased after the digestion of all mushroom preparations pointing to the potential impact of these preparation on the gut-brain axis and energy metabolism. Caco-2/THP1 co-culture studies with fermentations from Cordyceps, Reishi, and the 10 mushroom blend colonic simulations demonstrated significant improvement in intestinal barrier function with all test products. The results suggest that the mushroom preparations tested have prebiotic effects
Recommended Citation
Daoust, Julie, "Functional mushrooms supplementation as a prebiotic fibre impacting microbiome composition, the gut metabolome and impact on the intestinal epithelial barrier integrity" (2025). Oxford ICSB. 21.
https://egrove.olemiss.edu/icsb/2025_ICSB/Schedule/21
Publication Date
April 2025
Accessibility Status
Screen reader accessible, Searchable text
Included in
Functional mushrooms supplementation as a prebiotic fibre impacting microbiome composition, the gut metabolome and impact on the intestinal epithelial barrier integrity
Oxford Conference Center, Oxford MS
This study aimed to determine if functional mushroom preparation supplementation with Cordyceps militaris mycelium and fruiting body, Ganoderma lucidum mycelium and fruiting body, and a blend of ten species of functional mushrooms could impact gut health. The Ex Vivo model Simulator of Human Intestinal Microbial Ecosystem (SHIME®) has been used to study the behaviour of a human microbiome in response to various factors. The aim of this study was to demonstrate changes in the microbiome after supplementation with functional mushroom preparation and the resulting neuroactive metabolome using the SHIME® model. The endpoints measured were microbial fermentation activity, metabolic fingerprinting, microbial community composition, barrier integrity (TEER) and inflammation (pro- and anti-inflammatory cytokines) and targeted metabolomics evaluation of the compounds produced by the microbiome in the samples. The results showed that functional mushroom supplementation did change the composition of the gut microbiota. The type of species enriched in each experiment were unique to each mushroom preparation pointing to the unique composition of each mushroom preparations beyond their fiber content. Supplementation with all mushroom preparations led to an increase in SCFAs. SCFAs play a pivotal role in homeostasis and physiology in the gut but also in other organs. The analysis of the metabolome revealed increase concentration of unique molecules for each mushroom preparations although concentrations of butyrobetaine and tryptamine were increased after the digestion of all mushroom preparations pointing to the potential impact of these preparation on the gut-brain axis and energy metabolism. Caco-2/THP1 co-culture studies with fermentations from Cordyceps, Reishi, and the 10 mushroom blend colonic simulations demonstrated significant improvement in intestinal barrier function with all test products. The results suggest that the mushroom preparations tested have prebiotic effects
https://egrove.olemiss.edu/icsb/2025_ICSB/Schedule/21