Document Type

Oral Presentation

Location

Oxford Conference Center

Event Website

https://oxfordicsb.org/

Start Date

21-4-2026 2:20 PM

End Date

21-4-2026 2:40 PM

Description

We present metabolomic characterizations of Hericium erinaceus (Bull.) Pers. (lion’s mane, yamabushitake, etc.), and Laricifomes officinalis (Vill.) Kotl. & Pouzar (syn. Fomitopsis officinalis; agarikon, eburiko, etc.) fungal cultures by high resolution orbitrap mass spectrometry and HPLC-DAD. Primary focus has been applied to the cultivation of these species on brown rice, Oryza sativa L., as a solid substrate.   In the case of H. erinaceus, we have observed key differences in lipid metabolite profiles over a 28-day fermentation time series suggesting possible grain lipid feedstocks for fungal-derived lipid production, providing clear evidence of substrate biotransformation.   For L. officinalis cultures, we observe distinct triterpenoid diversity and have documented possible anthraquinones present across different strains and culture types evaluated.  Future work will continue to elucidate how metabolite profiles vary as a function of strain, substrate, and culturing conditions, and how these parameters can best be optimized across all functional fungi species of interest.

Comments

Dr. Kyle Meyer is a Research Scientist in the Research & Development Department at Fungi Perfecti, LLC. His work has focused on the characterization of natural products from fungal fruit bodies, liquid cultured mycelium, and mycelium grown on solid substrates. Kyle received a B.S. in Geology from the University of California, Davis in 2009, M.S. in Geological Sciences from the University of Texas, Austin in 2012, and his Ph.D. in Earth and Environmental Sciences at the University of Michigan, Ann Arbor in 2018.  His research began in biogeochemistry in application to past climate and environmental reconstructions. He worked as Assistant Laboratory Supervisor for PSI Labs LLC where he developed analytical cannabis services for the state of Michigan.  He was a post-doctoral research fellow at Portland State University from 2019 to 2022 where his research focused on biochemistry and mycology including fungal secondary metabolite expression.

We thank Paul Stamets, Steve Cividanes, and all our colleagues at Fungi Perfecti, LLC, with specific appreciation for the contributions of the other members of the R&D Department—Chase Beathard, Elizabeth Doar, and Jessica Kishiyama. We also credit the analytical contributions of David Gaul and Samuel Moore of the Systems Mass Spectrometry Core at Georgia Institute of Technology.

Dr. Kyle Meyer is a Research Scientist in the Research & Development Department at Fungi Perfecti, LLC.

His work has focused on the characterization of natural products from fungal fruit bodies, liquid cultured mycelium, and mycelium grown on solid substrates.

Kyle received a B.S. in Geology from the University of California, Davis in 2009, M.S. in Geological Sciences from the University of Texas, Austin in 2012, and his Ph.D. in Earth and Environmental Sciences at the University of Michigan, Ann Arbor in 2018. 

His research began in biogeochemistry in application to past climate and environmental reconstructions. He worked as Assistant Laboratory Supervisor for PSI Labs LLC where he developed analytical cannabis services for the state of Michigan. 

He was a post-doctoral research fellow at Portland State University from 2019 to 2022 where his research focused on biochemistry and mycology including fungal secondary metabolite expression.

Publication Date

April 2026

Accessibility Status

Screen reader accessible, Searchable text

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Apr 21st, 2:20 PM Apr 21st, 2:40 PM

Metabolomic characterizations of Hericium erinaceus (Bull.) Pers. and Laricifomes officinalis (Vill.) Kotl. & Pouzar fungal cultures

Oxford Conference Center

We present metabolomic characterizations of Hericium erinaceus (Bull.) Pers. (lion’s mane, yamabushitake, etc.), and Laricifomes officinalis (Vill.) Kotl. & Pouzar (syn. Fomitopsis officinalis; agarikon, eburiko, etc.) fungal cultures by high resolution orbitrap mass spectrometry and HPLC-DAD. Primary focus has been applied to the cultivation of these species on brown rice, Oryza sativa L., as a solid substrate.   In the case of H. erinaceus, we have observed key differences in lipid metabolite profiles over a 28-day fermentation time series suggesting possible grain lipid feedstocks for fungal-derived lipid production, providing clear evidence of substrate biotransformation.   For L. officinalis cultures, we observe distinct triterpenoid diversity and have documented possible anthraquinones present across different strains and culture types evaluated.  Future work will continue to elucidate how metabolite profiles vary as a function of strain, substrate, and culturing conditions, and how these parameters can best be optimized across all functional fungi species of interest.

https://egrove.olemiss.edu/icsb/2026_ICSB/Schedule/14