Posters and Spotlights
Trifluoromethyl Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides
Start Date
30-4-2025 11:30 AM
Document Type
Event
Description
Poster Presenter: Quadrat Yusuph
Research Team: Quadrat Yusuph, Sandeep K. Misra, Joshua S. Sharp
Abstract: Biomolecular footprinting is well-established for nucleic acids and proteins but lacks a method for carbohydrates. Glycan footprinting could enhance understanding of glycan interactions via mass spectrometry. We verified that CF3 labeled the model trisaccharide NAG3 across the oligosaccharide. We developed a quenching and reduction protocol for improved post-labeling analysis. Development of the first radical covalent footprinting method for complex carbohydrates.
Relational Format
poster
Recommended Citation
Yusuph, Quadrat, "Trifluoromethyl Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides" (2025). Showcase of Research and Scholarly Activity. 65.
https://egrove.olemiss.edu/ored_showcase/2025/posters/65
Trifluoromethyl Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides
Poster Presenter: Quadrat Yusuph
Research Team: Quadrat Yusuph, Sandeep K. Misra, Joshua S. Sharp
Abstract: Biomolecular footprinting is well-established for nucleic acids and proteins but lacks a method for carbohydrates. Glycan footprinting could enhance understanding of glycan interactions via mass spectrometry. We verified that CF3 labeled the model trisaccharide NAG3 across the oligosaccharide. We developed a quenching and reduction protocol for improved post-labeling analysis. Development of the first radical covalent footprinting method for complex carbohydrates.