Posters and Spotlights
Evaluation of glyceroglycolipids for in vitro antiviral activity against influenza A (H1N1)
Start Date
30-4-2025 11:30 AM
Document Type
Event
Description
Poster Presenter: Jin Zhang
Research Team: Jin Zhang, Suresh Chandra V. A. R. Annam, Ahmed E. B. A. Elbermawi, Jungmoo Huh, Zulfiqar Ali, Amar G. Chittiboyina, Ikhlas A. Khan, Nirmal D. Pugh
Abstract: The University of Mississippi Botanical Dietary Supplements Research Center is focused on evaluating the potential use of Limnospira (formally Arthrospira) to enhance resilience against influenza viral infection. Previously we reported that the fatty acid content on this botanical was significantly correlated with in vitro immune-enhancing activity [Toll-like receptor (TLR)2/TLR1 activation]. Since glyceroglycolipids are the major source of fatty acids in Limnospira, the objective of this study was to evaluate the in vitro antiviral activity of sixteen compounds. Seven of these were isolated from Limnospira [representing sulfoquinovosyl diacylglycerol (SQDG), sulfoquinovosyl monoacylglycerol (SQMG), monogalactosyl monoacylglycerol (MGMG) and sulfoquinovosyl glycerol (SQ-glycerol) classes), while nine were commercially obtained [representing monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG) and SQDG classes]. Antiviral activity was measured by assessing the inhibition of influenza A (H1N1) cytopathic effects on Madin-Darby canine kidney cells, with Ribavirin and Oseltamivir Acid as positive controls. Two compounds exhibited selective antiviral activity and belonged to the SQDG class (compound 7 exhibited an EC50 = 0.51µg/ml with a selective index > 200, compound 3 exhibited an EC50 = 6.5µg/ml with a selective index > 15).
Relational Format
poster
Recommended Citation
Zhang, Jin, "Evaluation of glyceroglycolipids for in vitro antiviral activity against influenza A (H1N1)" (2025). Showcase of Research and Scholarly Activity. 66.
https://egrove.olemiss.edu/ored_showcase/2025/posters/66
Evaluation of glyceroglycolipids for in vitro antiviral activity against influenza A (H1N1)
Poster Presenter: Jin Zhang
Research Team: Jin Zhang, Suresh Chandra V. A. R. Annam, Ahmed E. B. A. Elbermawi, Jungmoo Huh, Zulfiqar Ali, Amar G. Chittiboyina, Ikhlas A. Khan, Nirmal D. Pugh
Abstract: The University of Mississippi Botanical Dietary Supplements Research Center is focused on evaluating the potential use of Limnospira (formally Arthrospira) to enhance resilience against influenza viral infection. Previously we reported that the fatty acid content on this botanical was significantly correlated with in vitro immune-enhancing activity [Toll-like receptor (TLR)2/TLR1 activation]. Since glyceroglycolipids are the major source of fatty acids in Limnospira, the objective of this study was to evaluate the in vitro antiviral activity of sixteen compounds. Seven of these were isolated from Limnospira [representing sulfoquinovosyl diacylglycerol (SQDG), sulfoquinovosyl monoacylglycerol (SQMG), monogalactosyl monoacylglycerol (MGMG) and sulfoquinovosyl glycerol (SQ-glycerol) classes), while nine were commercially obtained [representing monogalactosyl diacylglycerol (MGDG), digalactosyl diacylglycerol (DGDG) and SQDG classes]. Antiviral activity was measured by assessing the inhibition of influenza A (H1N1) cytopathic effects on Madin-Darby canine kidney cells, with Ribavirin and Oseltamivir Acid as positive controls. Two compounds exhibited selective antiviral activity and belonged to the SQDG class (compound 7 exhibited an EC50 = 0.51µg/ml with a selective index > 200, compound 3 exhibited an EC50 = 6.5µg/ml with a selective index > 15).