Document Type

Oral Presentation

Location

Oxford Convention Center, 102 Ed Perry Boulevard Oxford, MS 38655

Event Website

https://oxfordicsb.org/

Start Date

16-4-2024 1:00 PM

End Date

16-4-2024 1:30 PM

Description

Nerium oleander is a well-known medicinal plant used in the Mediterranean region and traditional Indian medicine (Ayurveda) against a variety of diseases, including cancer. We investigated a cold-water extract of N. oleander leaves for their anticancer activities in vitro and in vivo. The cytotoxicity testing in the Oncotest panel of tumor cell lines and concomitant COMPARE analysis showed a high degree of similarity to mitosis-inhibiting compounds. We verified this hypothesis by treating tumor cells transfected with a tubulin-GFP fusion cDNA construct and confocal microscopy as well as a biochemical tubulin polymerization assay. N. oleander showed a similar inhibition of tubulin depolymerization as the control drug paclitaxel. Furthermore, the N. oleander extract inhibited the growth of human mammary cancer xenograft MAXF 401 alone (test/Control value 24%) in nude mice. In combination with paclitaxel, it effected a strong synergism in combination with paclitaxel resulting in complete remissions. A clinical phase I trial with breast or colon cancer patients showed tolerable side effects (fever, vomiting, nausea, diarrhea, fatigue). A subsequent clinical phase II trial with 300+ patients revealed tumor control rates (complete and partial remissions plus stabilizations) in a range of 8-70% in 18 different tumor types. Reference: Rashan LJ, Özenver N, Boulos JC, Dawood M, Roos WP, Franke K, Papasotiriou I, Wessjohann LA, Fiebig HH, Efferth T. Molecules. 2023;28(4):1871.

Comments

We gratefully acknowledge the stipend of the Sibylle Kalkhof-Rose-Foundation to J.C.B. and a stipend of the German Academic Exchange Service (DAAD) to N.Ö. Furthermore, we are grateful for the support of the Confocal Microscopy Facility of the Institute of Molecular Biology, Johannes Gutenberg University, Mainz, Germany

Publication Date

April 2024

Accessibility Status

Searchable text

Download

Share

COinS
 
Apr 16th, 1:00 PM Apr 16th, 1:30 PM

Cold-water extract of Nerium oleander leaves inhibits cancer: From bench to bedside

Oxford Convention Center, 102 Ed Perry Boulevard Oxford, MS 38655

Nerium oleander is a well-known medicinal plant used in the Mediterranean region and traditional Indian medicine (Ayurveda) against a variety of diseases, including cancer. We investigated a cold-water extract of N. oleander leaves for their anticancer activities in vitro and in vivo. The cytotoxicity testing in the Oncotest panel of tumor cell lines and concomitant COMPARE analysis showed a high degree of similarity to mitosis-inhibiting compounds. We verified this hypothesis by treating tumor cells transfected with a tubulin-GFP fusion cDNA construct and confocal microscopy as well as a biochemical tubulin polymerization assay. N. oleander showed a similar inhibition of tubulin depolymerization as the control drug paclitaxel. Furthermore, the N. oleander extract inhibited the growth of human mammary cancer xenograft MAXF 401 alone (test/Control value 24%) in nude mice. In combination with paclitaxel, it effected a strong synergism in combination with paclitaxel resulting in complete remissions. A clinical phase I trial with breast or colon cancer patients showed tolerable side effects (fever, vomiting, nausea, diarrhea, fatigue). A subsequent clinical phase II trial with 300+ patients revealed tumor control rates (complete and partial remissions plus stabilizations) in a range of 8-70% in 18 different tumor types. Reference: Rashan LJ, Özenver N, Boulos JC, Dawood M, Roos WP, Franke K, Papasotiriou I, Wessjohann LA, Fiebig HH, Efferth T. Molecules. 2023;28(4):1871.

https://egrove.olemiss.edu/icsb/2024_ICSB/Schedule/16