Document Type

Oral Presentation

Location

Oxford Conference Center, Oxford MS

Event Website

https://oxfordicsb.org/

Start Date

8-4-2025 1:30 PM

Description

Green tea extracts (GTEs) are common ingredients among dietary supplements marketed for weight loss and weight management. However, GTEs and their various catechin polyphenols have also been linked to a number of hepatotoxicity cases. The purpose of this study was to investigate, using the NZO/HlLtJ obese mouse model, the hepato- and cardiotoxic potential of a well-characterized GTE in combination with caffeine (CAF) moderate exercise (EX, 1 hour daily for the duration of 8 weeks) as well as its ability to promote weight loss. No evidence of hepato- or cardiotoxicity was noted when GTE was administered either alone or in combination with CAF and/or EX. While no effects on the body weights were observed in mice administered GTE alone, eight weeks of GTE administration in combination with CAF resulted in significant body weight reduction in obese mice, which was further enhanced by EX. Furthermore, GTE/CAF combinations partially mitigated obesity-associated small and large droplet steatosis and decreased both portal and lobular inflammation, demonstrating hepatoprotective capabilities. At the same time, no molecular changes associated with lipid metabolism were observed in the liver and fat tissue of experimental mice. Administration of GTE at doses comparable to those consumed by humans resulted in significant modulation of gut microflora, with increases in commensal and beneficial bacterial species. These favorable changes in the gut microbiome may provide a mechanistic link to weight loss management. Results of this study demonstrate that appropriate doses of caffeinated GTE can serve as a useful adjunct in weight management strategies. Furthermore, clinically relevant doses of GTE/CAF combinations did not produce hepato- or cardiotoxicity, but rather show significant potential for reversal of early signs of non-alcoholic fatty liver disease and hepatosteatosis.

Publication Date

April 2025

Accessibility Status

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Apr 8th, 1:30 PM

Effects of caffeinated green tea extract and exercise in obese NZO/HlLtJ mice

Oxford Conference Center, Oxford MS

Green tea extracts (GTEs) are common ingredients among dietary supplements marketed for weight loss and weight management. However, GTEs and their various catechin polyphenols have also been linked to a number of hepatotoxicity cases. The purpose of this study was to investigate, using the NZO/HlLtJ obese mouse model, the hepato- and cardiotoxic potential of a well-characterized GTE in combination with caffeine (CAF) moderate exercise (EX, 1 hour daily for the duration of 8 weeks) as well as its ability to promote weight loss. No evidence of hepato- or cardiotoxicity was noted when GTE was administered either alone or in combination with CAF and/or EX. While no effects on the body weights were observed in mice administered GTE alone, eight weeks of GTE administration in combination with CAF resulted in significant body weight reduction in obese mice, which was further enhanced by EX. Furthermore, GTE/CAF combinations partially mitigated obesity-associated small and large droplet steatosis and decreased both portal and lobular inflammation, demonstrating hepatoprotective capabilities. At the same time, no molecular changes associated with lipid metabolism were observed in the liver and fat tissue of experimental mice. Administration of GTE at doses comparable to those consumed by humans resulted in significant modulation of gut microflora, with increases in commensal and beneficial bacterial species. These favorable changes in the gut microbiome may provide a mechanistic link to weight loss management. Results of this study demonstrate that appropriate doses of caffeinated GTE can serve as a useful adjunct in weight management strategies. Furthermore, clinically relevant doses of GTE/CAF combinations did not produce hepato- or cardiotoxicity, but rather show significant potential for reversal of early signs of non-alcoholic fatty liver disease and hepatosteatosis.

https://egrove.olemiss.edu/icsb/2025_ICSB/Schedule/31